I-DNA / RNA Extraction

Intshayelelo yemveliso:

Isebenzisa itekhnoloji yokucoca intsimbi yemagnethi, iMagpure virus DNA/RNA kit yokuhlanjululwa inokukhupha iDNA/RNA yeentsholongwane ezahlukeneyo ezifana nentsholongwane ye-African Swine Fever kunye nenoveli coronavirus kwiisampulu ezahlukeneyo ezifana neserum, iplasma kunye nesisombululo sokuntywiliselwa kweswab, kwaye ingasetyenziswa kwiPCR esezantsi. / RT-PCR, ukulandelelana, uhlalutyo lwe-polymorphism kunye nolunye uhlalutyo lwe-nucleic acid kunye novavanyo lokubona. Ixhotyiswe nge-NETRACTION ngokuzenzekelayo isixhobo sokucoca i-nucleic acid kunye nekiti yokulayisha kwangaphambili, inokugqiba ngokukhawuleza ukutsalwa kwenani elikhulu leesampuli ze-nucleic acid.

Iimpawu zeMveliso:

1. Ikhuselekile ukusebenzisa, ngaphandle kwe-reagent enetyhefu
2. Kulula ukuyisebenzisa, akukho mfuneko yeProteinase K kunye ne-Carrier RNA
3. Khipha i-DNA ye-viral / i-RNA ngokukhawuleza nangempumelelo ngovakalelo oluphezulu
4. Ezothutho kunye nokugcina kwiqondo lokushisa legumbi.
5. Ilungele ukuhlanjululwa kwe-viral nucleic acid eyahlukeneyo
6. Ixhotyiswe nge-NUETRACTION ngokuzenzekelayo isixhobo sokuhlanjululwa kwe-nucleic acid ukwenza inkqubo ye-32 isampuli ngaphakathi kwe-30 min.