Ukukhupha i-DNA/RNA

Intshayelelo yemveliso:

Isebenzisa ubuchwepheshe bokucoca iintsimbi zemagnethi, ikhithi yokucoca i-DNA/RNA yegciwane leMagpure inokukhupha i-DNA/RNA yeentsholongwane ezahlukeneyo ezifana ne-African Swine Fever virus kunye ne-coronavirus entsha kwiisampuli ezahlukeneyo ezifana ne-serum, i-plasma kunye nesisombululo sokuntywiliselwa kwe-swab, kwaye ingasetyenziswa kwi-downstream PCR/RT-PCR, ukulandelelana, uhlalutyo lwe-polymorphism kunye nezinye iimvavanyo zohlalutyo lwe-nucleic acid kunye nokufumanisa. Ixhotyiswe nge-NETRACTION ngokuzenzekelayo ngokupheleleyo isixhobo sokucoca i-nucleic acid kunye nekhithi yokulayisha kwangaphambili, inokugqiba ngokukhawuleza ukukhupha inani elikhulu leesampuli ze-nucleic acid.

Iimpawu zeMveliso:

1. Kukhuselekile ukuyisebenzisa, ngaphandle kwe-reagent enobuthi
2. Kulula ukuyisebenzisa, akukho mfuneko yeProteinase K kunye neCarrier RNA
3. Susa i-DNA/RNA yentsholongwane ngokukhawuleza nangokufanelekileyo ngobuchule obuphezulu
4. Ukuthutha nokugcina kwindawo eshushu yegumbi.
5. Ifanelekile kwiindlela ezahlukeneyo zokucocwa kwe-nucleic acid yentsholongwane
6. Ixhotyiswe nge-NUETRACTION ngokuzenzekelayo ngokupheleleyo isixhobo sokucoca i-nucleic acid ukuze icubungule iisampulu ezingama-32 kwimizuzu engama-30.