I-DNA / RNA Extraction

Intshayelelo yemveliso:

Ukusebenzisa iteknoloji yokucoca i-bead magnetic, i-Magpure virus ye-DNA / i-RNA yokucoca ikiti inokukhupha i-DNA / i-RNA yeentsholongwane ezahlukeneyo ezifana ne-African Swine Fever virus kunye ne-coronavirus yenoveli kwiisampulu ezahlukeneyo ezifana ne-serum, i-plasma kunye nesisombululo sokuntywiliselwa kwe-swab, kwaye ingasetyenziswa kwi-PCR / RT-PCR esezantsi, ulandelelwano, uhlalutyo lwe-polymorphism kunye nolunye uhlalutyo lokufunyanwa kwe-nucleic acid. Ixhotyiswe nge-NETRACTION ngokuzenzekelayo isixhobo sokucoca i-nucleic acid kunye nekiti yokulayisha kwangaphambili, inokugqiba ngokukhawuleza ukutsalwa kwenani elikhulu leesampuli ze-nucleic acid.

Iimpawu zeMveliso:

1. Ikhuselekile ukusebenzisa, ngaphandle kwe-reagent enetyhefu
2. Kulula ukuyisebenzisa, akukho mfuneko yeProteinase K kunye ne-Carrier RNA
3. Khipha i-DNA ye-viral / i-RNA ngokukhawuleza nangempumelelo ngovakalelo oluphezulu
4. Ezothutho kunye nokugcina kwiqondo lokushisa legumbi.
5. Ilungele ukuhlanjululwa kwe-viral nucleic acid eyahlukeneyo
6. Ixhotyiswe nge-NUETRACTION ngokuzenzekelayo isixhobo sokuhlanjululwa kwe-nucleic acid ukwenza inkqubo ye-32 isampuli ngaphakathi kwe-30 min.