Qalabka lagu ogaanayo Asiidhka Nucleic-ka ee SARS-CoV-2 (Fluorescence RT-PCR)

Sars-CoV-2 Qalabka Lagu Ogaado Ashitada Nukliyeerka (Fluorescence RT-PCR) Sawirka

Sharaxaad Gaaban:

Xirmada ogaanshaha ee SARS-COV-2 nucleic acid (Fluorescence RT-PCR) waxaa loo isticmaali karaa ogaanshaha nucleic acid ee novel coronavirus, loo isticmaalo ogaanshaha kaabaya iyo baarista epid-emiological ee novel coronavirus infekshanka, ku habboon CDC, isbitaallada, shaybaarka caafimaadka qolo saddexaad, xarunta baaritaanka jirka iyo shaybaarada rugta kale.

NALA SOO XIRIIR

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1,Dareen sare: Xadka ogaanshaha (LoD)＜2×102 koobi/ml.

2, Hiddo-sidaha laba-geesoodka ah: Soo ogow hidda-wadaha ORFlab iyo hidda-wadaha N hal mar, u hoggaansan nidaamka WHO.

3, Ku habboon qalabyada kala duwan: ABI 7500/7500FAST; Roche LightCycler480; BioRad CFX96; BigFish-BFQP96/48 noo gaar ah.

4, Degdeg ah oo fudud: Reagent pre-mixed waa fududahay in la isticmaalo, macaamiishu waxay u baahan yihiin inay ku daraan enzyme iyo template. Xirmada soo saarista aashitada nucleic-ka ee Bigfish ayaa si fiican ugu habboon baaritaankan. Adigoo isticmaalaya mishiinka soo saarista tooska ah ee buuxa, way dhakhso badan tahay in la sameeyo muunado badan.

5, Badbaadada noolaha: Bigfish wuxuu bixiyaa Tusaalaha Dareeraha Preservative si uu si degdeg ah u hawlgeliyo fayraska si loo hubiyo badbaadada hawl-wadeennada.