News - Struggling with bacterial RNA extraction? Bige Feixu has the answer.

In microbiology research and bacterial detection, high-quality RNA serves as the cornerstone for downstream applications such as RT-PCR, qPCR, transcriptome sequencing, and Northern blotting. However, the complex structure of bacterial cell walls and the significant differences in lysis requirements between Gram-positive and Gram-negative bacteria—combined with the tedious nature and poor batch-to-batch reproducibility of manual extraction methods—often make this step the most time-consuming and error-prone part of the experimental workflow.

Product Highlight

BigeFeixu has launched the new BFMP26R Bacterial RNA Extraction Kit. Utilizing an optimized, unique buffer system and superparamagnetic beads that specifically bind RNA, the kit enables the rapid and efficient extraction and purification of total RNA from a wide range of Gram-negative and Gram-positive bacterial samples, providing a stable and reliable nucleic acid solution for bacteriology research and microbial detection.

I Bacterial RNA Extraction Kit

The BFMP26R kit demonstrates excellent sample versatility, accommodating both bacterial suspensions and colonies. It ensures stable extraction for both Gram-positive and Gram-negative bacteria, effectively meeting the requirements for processing standard microbial culture samples. To address the long-standing challenge of lysing Gram-positive bacteria—which possess thick cell walls and dense peptidoglycan layers—the kit includes a specialized lysozyme. Combined with an optimized lysis system, it effectively disrupts cell walls and fully releases intracellular RNA, ensuring consistent, high-efficiency extraction across diverse bacterial species and growth stages.

II. Product Features

01 Broad Sample Compatibility: Capable of extracting RNA from various Gram-negative and Gram-positive bacteria; compatible with both liquid cultures and bacterial colonies. Stable extraction is achieved with culture volumes up to 5 mL and bacterial loads up to 2 × 10⁹ cells.

02 High-Efficiency & Stable Extraction: Features an optimized lysis system combined with high-capacity magnetic beads, resulting in high RNA yields and clear, intact bands with no significant degradation.

03 Excellent Purity: A260/A280 ratios consistently range from 1.9 to 2.1, and A260/A230 ratios exceed 2.0. Residual protein and salt ions are minimal, making the product directly compatible with various downstream enzymatic reactions.

04 Safe & Non-Toxic: No toxic organic solvents (such as phenol or chloroform) are used during the process, ensuring operational safety and environmental friendliness.

05 Automation-Ready: Designed with pre-filled reagents, the kit is perfectly compatible with the BFEX series nucleic acid extractors from Bige Feixu. It enables one-touch, high-throughput extraction, saving labor and minimizing human error.

III. Compatible Instruments

BigFeixu BFEX-16E/BFEX-32/BFEX-32E/BFEX-96E

IV. Experimental Results

Extraction was performed using 1 mL of an overnight culture; the measured data are as follows:

Sample	Concentration (ng/μL)	A260/A280	A260/A230
Lactic acid bacteria (Gram-positive)	186.07	1.95	2.11
Lactic acid bacteria (Gram-positive)	195.43	1.98	2.06
Escherichia coli (Gram-negative)	224.00	2.10	2.22
Escherichia coli (Gram-negative)	224.83	2.08	2.19

V. Product Specifications

Product Name	Product Model	Specification
Magnetic Bead-Based Bacterial RNA Extraction & Purification Kit (Pre-filled)	BFMP26R	32T
Magnetic Bead-Based Bacterial RNA Extraction & Purification Kit (Pre-filled)	BFMP26R1	40T
Magnetic Bead-Based Bacterial RNA Extraction & Purification Kit (Pre-filled)	BFMP26R96	96T
Lysozyme	BFRD019	1 mL/vial (50 mg/mL)

Post time: Sep-17-2026