Qalabka lagu ogaanayo Asiidhka Nucleic-ka ee SARS-CoV-2 (Fluorescence RT-PCR)

Sars-CoV-2 Qalabka Lagu Ogaado Ashitada Nukliyeerka (Fluorescence RT-PCR) Sawirka

Sharaxaad Gaaban:

Qalabka ogaanshaha ee SARS-COV-2 nucleic acid (Fluorescence RT-PCR) waxaa loo isticmaali karaa ogaanshaha aashitada nucleic ee novel coronavirus, loo isticmaalo ogaanshaha kaaliyaha iyo baaritaanka epid-emiological ee caabuqa coronavirus novel, oo ku habboon CDC, isbitaallada, caafimaadka qolo saddexaad shaybaadhka, xarunta baadhista jidhka iyo shaybaadhada kale ee rugaha caafimaadka.

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1,Dareen sare: Xadka ogaanshaha (LoD)22×102 koobi/ml.

2, Hiddo-sidaha laba-geesoodka ah: Soo ogow hidda-wadaha ORFlab iyo hidda-wadaha N hal mar, u hoggaansan nidaamka WHO.

3, Ku habboon qalabyada kala duwan: ABI 7500/7500FAST;Roche LightCycler480;BioRad CFX96;BigFish-BFQP96/48 noo gaar ah.

4, Degdeg ah oo fudud: Reagent pre-mixed waa fududahay in la isticmaalo, macaamiisha kaliya waxay u baahan yihiin ku dar enzyme iyo template.Xirmada soo saarista aashitada nucleic-ka ee Bigfish ayaa si fiican ugu habboon baaritaankan.Adigoo isticmaalaya mishiinka soo saarista tooska ah oo buuxa, way dhakhso badan tahay in la sameeyo muunado badan.

5, Badbaadada noolaha: Bigfish wuxuu bixiyaa Tusaalaha Dareeraha Preservative si uu si degdeg ah u hawlgeliyo fayraska si loo hubiyo badbaadada hawl-wadeennada.