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According to the Information Office of the Ministry of agriculture and rural areas, in August 2018, an African swine plague occurred in Shenbei New District, Shenyang City, Liaoning Province, which is the first African swine plague in China. As of January 14, 2019, African swine plague has occurred in more than 20 provinces in China, killing 916000 pigs, causing public concern.
African swine fever (ASF)
ASF(African Swine fever) is a severe infectious disease caused by African swine fever virus (ACFV) infecting domestic pigs and wild boars (African wild boar, European wild boar, etc.). The world organization for animal health (OIE) has listed it as a statutory reported animal disease, which is also a kind of animal epidemic that China focuses on preventing.
Characteristics of the disease
The clinical manifestations are fever (up to 40 ~ 42 ℃), tachycardia, dyspnea, partial cough, serous or mucinous purulent secretion in eyes and nose, skin cyanosis, obvious bleeding of kidney, lymph node and gastrointestinal mucosa. The clinical symptoms of African classical swine fever are similar to those of classical swine fever, which can only be diagnosed by laboratory monitoring.
Solution to African classical swine fever
1. Sample processing
It is suitable for blood and various tissues of suspected sick pigs: spleen, lymph node and kidney tissues.
Blood samples
Take 200 μ L blood sample, 5000g centrifugal 5 min, take the supernatant for inspection.
Tissue samples
After the tissue samples were fully ground, appropriate amount of normal saline or PBS was added, and the supernatant was centrifuged to be tested.
2. Automatic extraction
Hangzhou Bigfish Bio-tech Co., Ltd. BFEX-32 automatic nucleic acid extractor can complete the extraction of 32 samples in 30 minutes, which can meet your requirements of extracting a large number of samples. The whole process does not need any other operation, saves time and reduces the error of manual operation, and improves the work efficiency as much as possible.
3. High purity nucleic acid purification
MagPure Nucleic acid extraction kit, with BFEX-32, the product completely matched PCR & QPCR detection.
4. Computer testing and analysis
According to the actual situation of the laboratory, the user adopts qualitative or quantitative detection scheme.
Hangzhou Bigfish Bio-tech Co., Ltd. provides a qualitative (Quantitative) detection kit for African classical swine fever (ACFV) with FC-96G (BFQP-16/48), which can detect ACFV extensively, sensitively and reliably.
More content, please pay attention to the official WeChat official account of Hangzhou Bigfish Bio-tech Co., Ltd.
Post time: May-23-2021
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