News - Accurate Microvolume Measurement, One Instrument for Multiple Applications – Bigfish BFMUV-3100 Series Microvolume Spectrophotometer

Rapid quantification and purity assessment of nucleic acids and proteins are fundamental and essential steps in molecular testing and life science research.

Core Quality Control Device

The BFMUV-3100 microvolume spectrophotometer, developed by Bigfish for life science and molecular detection laboratories, serves as a core quality control device linking sample purification and downstream amplification analysis.

• Key Capabilities

It enables fast concentration measurement and purity evaluation for nucleic acids, proteins, bacterial suspensions and other biological samples.

• Advanced Technology

With self-developed optical detection technology and user-friendly operation design, this instrument delivers stable, precise and efficient microvolume quantification solutions for laboratories.

It also complements Bigfish’s full product portfolio covering sample pre-treatment, nucleic acid extraction, amplification testing and concentration quality control.

Advanced Multi-Pathlength Detection

At the core of its performance, the BFMUV-3100 adopts motor-controlled multi-pathlength detection technology, which differs from conventional single or dual-pathlength instruments. It automatically switches among multiple pathlength settings including 0.01 mm, 0.02 mm, 0.05 mm, 0.2 mm and 1.0 mm.

This design supports sample detection from ultra-low to extremely high concentrations, with notable improvements in stability, repeatability and linear range. Thanks to its wide-range optical architecture, the measurable concentration range is over 150 times that of standard UV-Vis spectrophotometers.

Most samples can be tested directly without serial dilution. This eliminates tedious dilution steps and shortens testing time per sample. It also removes systematic errors caused by manual dilution. For precious clinical specimens and trace extracted products, it minimizes sample loss and the risk of cross-contamination during handling.

Pulsed Flash Technology & Sample Integrity

To protect sensitive biological samples, the instrument uses a pulsed flash light source. The flash duration is extremely short, and the total light exposure is far lower than traditional continuous light sources. This reduces the risk of nucleic acid degradation during testing and preserves sample integrity for subsequent experiments.

Cost Efficiency

The pulsed mode extends the service life of the light source and cuts long-term laboratory maintenance costs.

Zero Preheating

The instrument is ready for testing immediately after power-on, matching frequent, intermittent testing workflows.

This combination of features perfectly matches the demands of busy research and diagnostic labs where efficiency and accuracy are paramount.

Comprehensive Detection Modes

Rich detection modes cover most routine quantification requirements in molecular labs. It supports concentration and purity analysis for dsDNA, ssDNA, total RNA, oligonucleotides and other nucleic acid samples, as well as native and labelled proteins.

After each test, the system automatically calculates key purity ratios such as A260/A280 and A260/A230, helping researchers quickly evaluate sample quality for downstream PCR, qPCR, sequencing and restriction digestion.

Microbiology & Synthetic Biology

For microbiology and synthetic biology workflows, it features an exclusive OD600 optical channel. Combined with cuvette mode, it monitors the concentration of bacterial and yeast cultures. Researchers can track cell growth status accurately for induction, strain passaging and growth curve plotting. One single instrument fulfils quantification needs for both molecular biology and microbiology, saving laboratory budget and bench space.

Advanced Fluorescence Capability

For users facing low-concentration trace samples, the upgraded BFMUV-3100F variant adds fluorescence detection capability. Paired with dedicated fluorescence reagents, it achieves highly specific quantification through binding between fluorescent dyes and target nucleic acids.

The limit of detection for dsDNA reaches 0.5 pg/μL.

Compared with standard UV absorbance methods, fluorescence quantification avoids interference from free nucleotides and protein impurities. It is ideal for NGS library preparation, single-cell nucleic acid samples and limited clinical specimens, greatly expanding the application scope of the platform.

The BFMUV-3100 is built for practical lab operation. It runs on an in-house developed Android system with a 7-inch high-definition capacitive touchscreen. The full testing workflow is operated directly on the touch panel, with no external computer required. Standalone operation makes it suitable for space-limited labs, biosafety cabinets and mobile testing setups.

To simplify data traceability, a built-in thermal printer generates complete test reports with one click, which is convenient for teaching labs and diagnostic facilities.

All test data can also be exported via USB for sorting, archiving and further analysis.

The compact, lightweight body fits easily on any lab bench. The sample pedestal is made of corrosion-resistant material and only requires simple wiping after use, effectively preventing cross-contamination during high-throughput daily testing.

As a key component of Bigfish’s integrated molecular biology ecosystem, the BFMUV-3100 microvolume spectrophotometer works seamlessly with the brand’s automatic nucleic acid extractors, thermal cyclers, real-time qPCR systems and magnetic bead-based extraction kits. Every stage from sample lysis, purification, QC quantification to amplification detection is supported by self-developed Bigfish products. Consistent parameter matching across the workflow and unified technical support bring extra convenience to end users.

This instrument has been widely deployed in university research institutes, clinical diagnostic labs, food and agricultural testing facilities and microbiology enterprises. It delivers reliable quantitative data for routine molecular cloning, gene expression analysis, pathogen detection and microbial monitoring. With robust optical performance, comprehensive functions and competitive pricing, the BFMUV-3100 is a practical choice for basic quantification. It also reflects Bigfish’s commitment to developing complete workflow solutions tailored to the full demands of molecular diagnostics.

Post time: Nov-04-2026