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Magnetic Bead Method Efficiently Solves Challenges in Environmental Water DNA Extraction
In fields such as environmental microbiology research and water pollution monitoring, the extraction of high-quality genomic DNA is a critical prerequisite for downstream applications including PCR/qPCR and next-generation sequencing (NGS). However, environmental water samples are highly complex, containing diverse microbial communities, hard-to-lyse strains such as Gram-positive bacteria, and long-standing challenges associated with traditional extraction methods—such as the use of toxic reagents and complicated procedures—which have continuously troubled researchers.
Now, Bigfish Sequencing introduces the BFMP24R Magnetic Bead-Based Environmental Water Genomic DNA Extraction and Purification Kit, which provides a comprehensive solution to these challenges through innovative technology and user-friendly design.
Product Overview
This kit is based on an optimized buffer system combined with high-performance nano magnetic beads. Genomic DNA specifically binds to functional groups on the bead surface and is separated under an external magnetic field. After multiple gentle washing steps to remove proteins, salts, and other impurities, high-purity genomic DNA is finally eluted.
Specifically designed for environmental water samples, the kit efficiently extracts bacterial DNA collected on filter membranes, including Gram-negative and Gram-positive bacteria (up to 2 × 10⁹ bacterial cells per single filter membrane). It is compatible with fully automated nucleic acid extraction systems for high-throughput processing. The extracted DNA is of consistent quality and can be directly used for PCR/qPCR, NGS, and other downstream applications.
Product Features
1. Broad-Spectrum Bacterial Extraction Capability
Efficiently extracts both Gram-negative and Gram-positive bacteria from water samples, covering microbial communities commonly found in freshwater and marine environments, and meeting diverse analytical requirements.
2. High Purity and High Yield
Delivers DNA with high purity, free of inhibitory contaminants, and stable yields suitable for direct downstream molecular applications.
3. Automated and High-Efficiency Compatibility
Fully compatible with Bigfish automated nucleic acid extraction systems, supporting simultaneous processing of 32 or 96 samples, significantly reducing processing time and improving laboratory efficiency.
4. Safe and User-Friendly Operation
No need for toxic organic reagents such as phenol or chloroform, minimizing laboratory safety risks. Core reagents are pre-packaged in 96-well plates, reducing manual pipetting errors and simplifying workflows.
Compatible Instruments
Bigfish BFEX-16E
BFEX-32
BFEX-32E
BFEX-96E
Experimental Results
A 600 mL river water sample was filtered through a membrane, and DNA was extracted using the Bigfish magnetic bead-based environmental water genomic DNA extraction and purification kit together with the compatible instrument. The extracted DNA was subsequently analyzed by agarose gel electrophoresis.
M: Marker1, 2: River water samples
Product Specifications
Post time: Dec-18-2025
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